Biological Imaging Based on Stimulated Raman Scattering
نویسندگان
چکیده
منابع مشابه
Vibrational imaging based on stimulated Raman scattering microscopy
A stimulated Raman scattering microscope with near-infrared picosecond laser pulses at high repetition rates (76MHz) and radio-frequency lock-in detection is accomplished. Based on stimulated Raman loss detection, we demonstrate noninvasive point-by-point vibrational mapping of chemical and biological samples with high sensitivity and without the requirement for labeling of the sample with natu...
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The SRS of a short laser pulse is important in the contexts of particle acceleration2 and inertial confinement fusion.3,4 In previous studies of the spatiotemporal evolution of SRS,5–13 the Stokes waves were allowed to pass freely through the pulse boundaries. However, the radial ponderomotive force associated with the pulse can expel plasma from the neighborhood of the pulse axis, in which cas...
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Hyperspectral stimulated Raman scattering (SRS) imaging has rapidly become an emerging tool for high content analyses of cell and tissue systems. The label-free nature of SRS imaging combined with its chemical specificity allows in situ and in vivo biochemical quantification at submicrometer resolution without sectioning and staining. Current hyperspectral SRS data analysis methods are based on...
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Optical imaging in vivo with molecular specificity is important in biomedicine because of its high spatial resolution and sensitivity compared with magnetic resonance imaging. Stimulated Raman scattering (SRS) microscopy allows highly sensitive optical imaging based on vibrational spectroscopy without adding toxic or perturbative labels. However, SRS imaging in living animals and humans has not...
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Stimulated Raman scattering (SRS) microscopy is a vibrational imaging platform developed to visualize chemical content of a biological sample based on molecular vibrational fingerprints. With high-speed, high-sensitivity, and three-dimensional sectioning capability, SRS microscopy has been used to study chemical distribution, molecular transport, and metabolic conversion in living cells in a la...
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ژورنال
عنوان ژورنال: Seibutsu Butsuri
سال: 2014
ISSN: 0582-4052,1347-4219
DOI: 10.2142/biophys.54.311